Aloe vera leaf gel, a new approach to enhance plant tissue culture

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Volume 82, Article 4

[Volume 82, Article 4]

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I. HAMDENI¹

S. SLIM²

A. BOULILA³

T. BETTAIEB¹

¹Research Laboratory of Horticultural Sciences, National Agronomic Institute of Tunisia, University of Carthage, Tunisia;

²Research Unit of Biodiversity and Valorization of Resources in Mountainous Areas, School of Higher Education in Agriculture of Mateur, University of Carthage, Tunisia;

³Laboratory of Natural Substances, National Institute of Research and Physico-chemical Analyses, Biotechpole of Sidi Thabet, Ariana, 2020, Tunisia.

Abstract - This study investigates the nutritive properties of Aloe vera leaf gel (AvG) used in in vitro propagation from shoot tip explants of A. vera plant. Therefore, induced shoots of A. vera were prolifired on MS medium (Murashige and Skoog, 1962) supplemented with various concentrations of AvG as organic supplement. The highest shoot multiplication was found on M3 containing MS media + 0.2 mg/L IBA (β-indole butyric acid) + 3 mg/L BA(benzylamino-purine) + 50 g/L AvG (13.27 ±5.11). This demonstrated that adding 50 g/L AvG to the culture media has increased the number of shoots compared to the control treatment M1 (5.00 ±2.27). Moreover, the longest shoot (2.50 ±0.89 cm) was found on M2 medium (MS + 0.2 mg/L IBA + 3 mg/L BA + 25 g/L AvG) followed by M3 (50 g/L AvG) and M1 (control) respectively. For microshoots rooting, the effect of the substitution of MS medium by AvG was assessed. The AvG was used as a substitute source of nutrients to the culture media. The maximum number of roots (5.93 ±1.39; 5.73 ±1.75) and the longest root (6.20 ±1.32; 5.90 ± 1.43 cm) were recorded on control medium (Full MS) and 1/2 strength MS medium supplemented with 10% AvG. All of the rooted explants (100%) survived during and after the acclimatization in the pots in the culture room. The regenerated plants looked healthy and they were morphologically similar to the mother plants.

Keywords: in vitro propagation, shoot tip explants, Aloe vera leaf gel, substitution.